Esculetin inhibits cancer cell glycolysis by binding tumor PGK2, GPD2, and GPI

The target proteins phosphoglycerate kinase 2 (PGK2), glycerol-3-phosphate dehydrogenase (GPD2)GPD2 and glucose-6-phosphate isomerase (GPI) were screened by combining transcriptome, proteomics and reverse docking We detected the binding constant of the active compound using microscale thermophoresis (MST). It was found that esculetin bound well with three potential target proteins. The HepG2 cells were inoculated into 6-well plates at a concentration of 5 x 105/mL. There are two groups: HepG2 cells without esculetin, and HepG2 cells containing esculetin