Did diversity of PBI-DdeI satellite DNA in snakes correlate with rapid independent evolution and different functional roles?

we sought to improve our understanding of PBI-DdeI evolutionary mode in snake lineages comprising 40 snake species using dot-blot hybridization and PCR based approaches. Genome organization of the repeats was also examined by Southern blot hybridization and PCR approach. Various DNA fragments of PBI-DdeI were molecularly cloned from snakes to determine their nucleotide sequences. Quantification of PBI-DdeI was performed on different snake species using quantitative real-time polymerase chain reaction (qPCR) and determined in silico for copy number of the repeats on nine snake genome sequences. Chromosomal distribution of the satDNA was subsequently identified in snakes. This allowed us to delineate evolutionary dynamics of PBI-DdeI and investigate its significance.