Treating pain in sickle cell disease by means of focused ultrasound neuromodulation (Ultrasound Characterizations: <i>Ex-vivo</i> Scanning)

We developed a novel transcranial focused ultrasound transducer for treating pain in humanized sickle cell mice. The project promises to provide non-pharmacologic chronic pain treatment for patients associated with underlying conditions such as cancer or sickle cell disease (SCD).This dataset includes 4 original ex-vivo scans formatted in MATLAB workspace. Their long file names include the scanning senarios, power source levels, fundamental frequency, sampling number, pulse repetition frequency, cycle per pulse number, x and y distances with step size, z distance with slice size, and z focal distance.For convenience, we also attached other 4 .mat files with formatted transcranial focused ultrasound pressure magnitude data scanned through hydrophone in a water tank (3-dimensional variable: <b><i>I</i></b><b><i>ntensity_locations</i></b>). Each file name is labeled with the scanning scenarios, i.e., free water vs. mouse skull, and with voltage levels of the Vantage system, i.e., 2.4 V or 20 V. 2.4 V power source is the major voltage used to test the efficacy of tFUS stimulation in modulating pain, and the 20 V power source is the scaled-up scenario used in positive control condition to test the safety of tFUS stimulation in mice. The spatial step sizes of the 3D matrix (<b><i>Intensity_locations</i></b>) are included in each of these files (variables: <b><i>step </i></b>for x and y dimensions, <b><i>slice </i></b>for z dimension, physical unit: mm). The pulse duration (<b><i>PD</i></b>) is 200 micro-second. The variables <b><i>xaxis_locations</i></b>, <b><i>y</i></b><b><i>axis_locations</i></b>, and <b><i>z</i></b><b><i>axis_locations </i></b>described the total step/slice numbers along those three directions.The ex-vivo scannings were implemented in the presence of a freshly excised mouse skull sample. This work was supported in part by NIH EB029354, NS124564, and NS131069. More information can be found in the corresponding manuscript:Kim MG*, Yu K*, Yeh C-Y, Fouda R, Argueta D, Kiven S, Ni Y, Niu X, Chen Q, Kim K, Gupta K, He B. Low-intensity transcranial focused ultrasound changes pain-associated behaviors by modulating pain processing brain circuits. bioRxiv: https://doi.org/10.1101/2022.12.07.519518 (*Equal contribution, first author)If anyone uses the whole or part of the dataset provided here, we kindly ask you to cite the above publication in any of your publications or presentations: