Rhinogobio ventralis genome and annotation

The endangered fish<i> Rhinogobio ventralis</i> is endemic to the upper Yangtze River, China. Here, we generated a high-quality chromosome-level genome assembly of <i>R. ventralis</i> using PacBio and Hi-C techniques. The PacBio sequencing technology resulted in an assembly of 1039.34 Mb, with a Contig N50 size of 18.1 Mb, and containing 659.3 Mb of repeats. Hi-C data was utilized for chromosome assembly and 25 chromosome sequences were ultimately yielded. A total of 23,246 protein-coding genes were predicted with an average intron length of 2109.5 bp. Evolutionary analysis revealed that <i>R. ventralis</i> was closely related to <i>C. guichenoti</i> and they phylogenetically diverged from common ancestor ~23.5 million years ago (Mya). Comparative genomics analysis revealed 245 expended gene families in <i>R. ventralis</i>. Furthermore, positively selected genes identified in <i>R. ventralis</i><i> </i>primarilyfunction in DNA replicationand repair mechanisms. This genome will serve as a valuable resource for the future study of genomics, biology and adaptive evolution in <i>R. ventralis</i>.