Diet-dependent induction of life-history changes in Daphnia: 5α-cyprinol sulfate is not the kairomone

Daphnia responds to chemical cues released by fish with adaptive changes in behavior (DVM), morphology, and life history traits, e. g. a reduction in size at first reproduction (SFR). For cyprinid fish, it has been revealed that DVM and changes in morphology of Daphnia are induced by the bile salt 5α-cyprinolsulfate (CPS), while the substance that induces life-history changes is unknown. Here we show that C18-solid phase extracts from fish incubation water of roach (Rutilus rutilus) that have fed Daphnia (RfD) induce a reduction of SFR in D. magna while similar extracts with chironomid larvae as prey do not. Subsequent fractionation of RfD by HPLC revealed that the major bile salts of cyprinid fish, CPS and taurochenodeoxycholic acid, eluted outside the active fractions, while taurocholic acid (TCA) had its peak within the active fractions. A dose-response experiment revealed that up to 2 µM TCA did not reduce SFR, which excludes TCA as a potential kairomone. In conclusion, we show that..., , # Data from: Diet-dependent induction of life-history changes in Daphnia: 5α-cyprinol sulfate is not the kairomone

Dataset DOI: [10.5061/dryad.rr4xgxdkq](10.5061/dryad.rr4xgxdkq)

## Description of the data and file structure

Diet-dependent induction of life-history changes in *Daphnia*: 5α-cyprinol sulfate is not the kairomone

The Excel dataset 'Diet-dependent induction of life-history changes in *Daphnia*: 5α-cyprinol sulfate is not the kairomone' contains the full set of raw data. The individual experiments are separated by tabs and named after the figures. Abbreviations are listed within the Excel sheets. Also, unformatted CSV files for each figure are included for the analysis.

### Files and variables

Life-history experiments

**Figure 1:**

Life-history data of the RfD interaction with a C18-cartridge. The table represents measurements of Size at First Reproduction (SFR)—expressed in millimeters—for different experimental treatments that interact with a C18 cartridge. Each tr...,