N1-methylpseudouridine enhances immunogenicity of RNA/lipidnanoparticle vaccines targeting SARS-CoV-2 spike and the model antigen ovalbumin

The recent success of RNA/lipid nanoparticle (LNP)-based vaccine formulations in the clinic against SARS-CoV-2 and RSV has highlighted the potential of this platform.  While RNA-LNP vaccines share the fundamental principle of using lipid nanoparticles to deliver RNA molecules into host cells to induce antigen expression and antigen-specific immune responses, several factors including RNA type (i.e., messenger, self-amplifying), RNA chemistry and/or nanoparticle formulation can influence the vaccine’s stability, tolerability and overall activity.  Although these differences may influence clinical performance, the lack of head-to-head comparative studies limits the ability to identify which factors have contributed most to the success of the clinically approved vaccines. Herein, we evaluated the influence of N1-methylpseudouridine incorporation into the RNA molecule on the overall immunogenicity of RNA vaccines encoding either the pathogen derived antigen SARS-CoV-2 spike or the..., , # N1-methylpseudouridine enhances immunogenicity of RNA/lipidnanoparticle vaccines targeting SARS-CoV-2 spike and the model antigen ovalbumin

Dataset DOI: [10.5061/dryad.g79cnp64d](https://doi.org/10.5061/dryad.g79cnp64d)

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\# Data from: N1-methylpseudouridine Enhances Immunogenicity of RNA/Lipid Nanoparticle Vaccines Targeting SARS-CoV-2 Spike and the Model Antigen Ovalbumin

This README.md file was generated on 2025-12-18 by Bassel Akache.

GENERAL INFORMATION

1.  Title of Dataset: N1-methylpseudouridine Enhances Immunogenicity of RNA/Lipid Nanoparticle Vaccines Targeting SARS-CoV-2 Spike and the Model Antigen Ovalbumin

    Related Article of the same title submitted to Scientific Reports.

2.  Author information:

    Hiva Azizi^1a^, Tyler M. Renner^1a^, Gerard Agbayani^1^, Renu Dudani^1^, Blair A. Harrison^1^, Martin A. Rossotti^2^, Jamshid Tanha^2,3,4^, Michael J. McCluskie^1^, Felicity C. Stark^1^ and Bassel Akache^1*^

 

1Human He...,