Kruppel-like factor 5 is Required for Villus Formation and Terminal Differentiation of the Intestinal Epithelium

To identify putative KLF5 target genes that may mediate villus morphogenesis and epithelial maturation, we performed microarray analysis on intestinal samples isolated on E14.5, prior to the initiation of villus formation. Total RNA was isolated from dissected intestine segments located ~1.5 cm anterior to the cecum from E14.5 Klf5D/D and Klf5wt/wtShhEGFP/Cre+ embryos (n=3 samples/genotype, each sample a pool of two intestines). Gene expression profiles were compared using Affymetrix Mouse Gene 1.0 ST Array. Genes involved in the regulation of "epithelial cell differentiation/maturation", "cell adhesion," and "lipid synthesis, metabolism, and localization." Consistent with the lack of terminally differentiated cell types in the intestines of E18.5 Klf5D/D fetuses. To identify putative KLF5 target genes that may mediate villus morphogenesis and epithelial maturation, we performed microarray analysis on intestinal samples isolated on E14.5, prior to the initiation of villus formation. Total RNA was isolated from dissected intestine segments located ~1.5 cm anterior to the cecum from E14.5 Klf5D/D and Klf5wt/wtShhEGFP/Cre+ embryos (n=3 samples/genotype, each sample a pool of two intestines). Gene expression profiles were compared using Affymetrix Mouse Gene 1.0 ST Array.为鉴定可介导绒毛形态发生与上皮细胞成熟的潜在Krüppel样因子5（KLF5）靶基因，我们于绒毛形成启动前的胚胎第14.5天（E14.5）分离肠道样本并开展微阵列分析。我们从E14.5时期的Klf5D/D与Klf5wt/wtShhEGFP/Cre+胚胎中，分离盲肠前方约1.5 cm处的解剖肠道段，提取总RNA；每组基因型设置3个生物学重复样本，每个样本由2个肠道混合制备而成。采用Affymetrix小鼠基因1.0 ST芯片（Affymetrix Mouse Gene 1.0 ST Array）对两组的基因表达谱进行比较分析。本分析聚焦于参与“上皮细胞分化/成熟”“细胞黏附”以及“脂质合成、代谢与定位”调控的基因，该结果与E18.5 Klf5D/D胎鼠肠道中缺乏终末分化细胞类型的表型相符。 为鉴定可介导绒毛形态发生与上皮细胞成熟的潜在Krüppel样因子5（KLF5）靶基因，我们于绒毛形成启动前的E14.5时期分离肠道样本并开展微阵列分析。我们从E14.5时期的Klf5D/D与Klf5wt/wtShhEGFP/Cre+胚胎中，分离盲肠前方约1.5 cm处的解剖肠道段，提取总RNA；每组基因型设置3个生物学重复样本，每个样本由2个肠道混合制备而成。采用Affymetrix小鼠基因1.0 ST芯片（Affymetrix Mouse Gene 1.0 ST Array）对两组的基因表达谱进行比较分析。