Characterizing senescent lung epithelial cells

We characterized senescent alveolar epithelial cells in an effort to better understand how these cells contribute to lung remodeling and fibrosis. The transcriptional profiles of several different types of senescent lung epithelial cells was evaluated using various induction methods in order to better understand core characteristics associated with the senescent program in epithelial cells of the lung. Overall design: Three separate cell types have been subjected to hydrogen peroxide or doxorubicin treatment in order to induce senescence, as evidenced by positive staining for senescent-associated beta-galactosidase activity. This gave us the opportunity to look at senescence in different cell types and with differing methods of induction.