SNB-1::GFP Expression by D Motor Neurons.

Genetic analysis of tubulin mutant synaptic defects based on expression pattern of the synaptic vesicle marker Synaptobrevin::GFP (SNB-1::GFP). Young adult worms were scored for the number of SNB-1::GFP puncta in the ventral and dorsal nerve cords. Animals carrying the ju89 mutant chromosome in trans to a wild-type (+/ju89) or deficiency (Df9/ju89) chromosome or the tba-1 deletion allele (ok1135/ju89) exhibit partial defects. Deficiency/+ and ok1135/+ heterozygotes appear wild-type. Animals that express an extrachromosomal array containing tba-1 amplified from ju89 animals, Extba-1(R414) resemble ju89 mutants. Animals homozygous for the null tbb-2 allele (gk207) have a similar decrease in total SNB-1::GFP as single mutants and in combination with tba-1(ju89). Mean ± sd.*p**Synapse loss is strongly enhanced in tba-1(ju89); tbb-1 (gk207) animals due to loss of axons in the dorsal and ventral nerve cord.