Interaction identification between protein kinase MeSnRK2.12 and transcription factor MebHLH1 and its relative expression level in cassava Data

The stem segments of cassava variety var.SC8, which was planted in Dafeng Farm, Chengmai, Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, were grown for 90 days under natural light conditions, and subjected to 20% PEG6000 simulated drought treatment and 1.0 μ Mol L-1 (low concentration), 10.0 μ Mol L-1 (medium concentration) and 100.0 μ Mol L-1 (high concentration) ABA treatment, three biological repeats in each group, liquid nitrogen temporary storage after treatment, and then transferred to - 80 ° C low temperature storage for subsequent test. Quantitative primers were designed with MeSnRK2.12 CDS sequence (Table 1), and MonAmp ™ ChemoHS qPCR Mix (Mona Biotechnology Co., Ltd.), quantitative fluorescent PCR analysis of MeSnRK2.12 through the fluorescence quantitative PCR instrument (Biometra TA advanced 96G), cassava β- The tubulin gene is an internal reference. The relative expression was calculated by 2 - ∆ ∆ computerized tomography, and the Duncan method was used on IBM SPSS Statistics 21.0 software, with a confidence interval of 95%. The difference significance analysis was performed on the data in each treatment group. The data is significant if it contains different letters, and each sample has 3 duplicates. The MeSnRK2.12 protein sequence was amplified by PCR and connected to pSL1plus [22], pGBKT7, pNC-BiFC-Enn [23]. The subcellular localization experiment confirmed the position of the gene in the cell. The interaction between MeSnRK2.12 and bHLH68 was verified by yeast two-hybrid and bimolecular fluorescence complementation.