Replication Data for: Super-resolution imaging of Schlemm’s canal using 3D-SIM

Series of three-dimensional structured illumination microscopy (3D SIM) and deconvolution microscopy data of 3µm think tissue sections of Schlemm’s Canal in the chamber angle of mice’s eyes. Schlemm’s Canal endothelium was labeled with an anti-CD31 antibody and an AF647 secondary antibody, the slides were also treated with the nuclear stain DAPI. In addition, eye tissue has significant autofluorescence. The eyes are of either a CD1 mouse strain wildtype animal or of the same background but carrying a βB1-CTGF transgenic construct. CTGF is known to play a key role in mediating the effects of primary open angle glaucoma. The aim of the study was to test the feasibility of 3D SIM of eye tissue and especially in the examination of Schlemm’s Canal.

本数据集涵盖小鼠眼前房角施莱姆管（Schlemm’s Canal）3微米厚组织切片的三维结构照明显微镜（3D SIM）与反卷积显微镜成像数据。实验中，研究人员以抗CD31抗体及AF647标记二抗对施莱姆管内皮细胞进行荧光标记，同时使用细胞核染料DAPI对组织玻片完成染色。此外，眼部组织存在显著自发荧光。
实验所用眼部组织均来源于CD1小鼠品系，分为两类：野生型个体，以及同一遗传背景但携带βB1-CTGF转基因构建体的个体。已知结缔组织生长因子（CTGF）在原发性开角型青光眼的效应介导过程中发挥关键作用。本研究旨在验证眼部组织（尤其是施莱姆管）的三维结构照明显微镜成像可行性。