The PGR occupancy profiles in nonpregnant and gravid human myometrial tissues

Profiles of PGR genome occupancy in human myometrial specimens were documented by ChIP-seq to investigate downstream targets of the progesterone receptor and candidate partner transcription regulators at the nonpregnant and term pregnant stages. Overall design: Two nonpregnant and two term pregnant myometrial specimens were subject to ChIP-seq analysis. Viable tissue is taken from myometrial margins at the time of hysterotomy performed for clinically indicated cesarean section. After washing each specimen briefly in phosphate buffered saline (PBS), pieces of myometrium are flash frozen. All specimens are stored at <-80°C until use. ChIP-seq was performed by the Active Motif.