The data of RPhC, TLC, and MALDI analysis of molecular masses of fluorescent oligopeptides forming after incubation of X-OP21 with sle-IgGmix.
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*Free fluorescent compound; all analyzed OPs contained fluorescent X-component.**The same products of the hydrolysis separated by RPhC (Fig. 3A) were revealed in several peaks by MALDI spectrometry; the main peaks are marked in bold, while additional peaks containing low amount of the same OPs are shown in parentheses. C1 reflects the presence of signal corresponding to the analyzed product in spectrum of total reaction mixture.***The same several products of the hydrolysis corresponding to each peak after RPhC (Fig. 3A) were revealed not only by MALDI spectrometry, but also by TLC (Fig. 3B).§The relative content of different products after incomplete hydrolysis of X-OP21 was performed taking into account the data of TLC analysis (Fig. 3B); a range of the values from three repeats is given.
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2015-12-02



