Dynamical forces drive cell and organ morphology changes during embryonic development

Cells, tissues, and organs must change shape in precise ways during embryonic development to execute their functions. Multiple mechanisms including biochemical signaling pathways and biophysical forces help drive these morphology changes, but it has been difficult to tease apart their contributions, especially from tissue-scale dynamic forces that are typically ignored. We use a combination of mathematical models and in vivo experiments to study a simple organ in the zebrafish embryo called Kupffer’s vesicle. Modeling indicates that dynamic forces generated by tissue movements in the embryo produce shape changes in Kupffer’s vesicle that are observed during development. Laser ablations in the zebrafish embryo that alter these forces result in altered organ shapes matching model predictions. These results demonstrate that dynamic forces sculpt cell and organ shape during embryo development., , # Dynamical forces drive cell and organ morphology changes during embryonic development

Dataset DOI: [10.5061/dryad.w9ghx3g2h](10.5061/dryad.w9ghx3g2h)

## Description of the data and file structure

The details of the experiments are provided in the paper. 

### Files and variables

#### File: Fig-control-snapshots-speed-both.png

**Description:** KV middle plane and the corresponding lumen boundary for the computation of KV speed in unablated control experiments.

#### File: Fig-nc-snapshots-speed-both.png

**Description:** KV middle plane and the corresponding lumen boundary for the computation of KV speed in notochord ablation experiments.

#### File: Fig-pc-snapshots-speed-both.png

**Description:** KV middle plane and the corresponding lumen boundary for the computation of KV speed in posterior cells ablation experiments.

#### File: Fig-control-ablation-snapshots-shape-both.png

**Description:** KV middle plane and the corresponding lumen boundary for the computation of lumen sh...,