five

ATAC-seq analyses during spermatogenesis

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102954
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To determine the dynamics of open chromatin at a genomic resolution during spermatogenesis, we performed ATAC-seq and detected genomic regions of accessible chromatin by Tn5 transposase during spermatogenesis. We analyzed four representative stages of spermatogenesis: Thy1+ undifferentiated spermatogonia, which contains spermatogonial stem cells and progenitor cells; c-Kit+ differentiating spermatogonia from P7 testes; purified pachytene spermatocytes (PS) undergoing meiosis; and postmeiotic round spermatids (RS) from adult testes ATAC-seq analyses using purified spematogenic cells from wild-type mice and Scml2 knockout mice
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2021-07-25
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