Specialisation and plasticity in a primitive social insect (RNA-seq)

Biological systems not only have the remarkable capacity to build and maintain complex spatio-temporal structures in noisy environments, they can also rapidly break up and rebuild such structures. How such systems can simultaneously achieve both robust specialisation and plasticity is poorly understood. Here we use primitive societies of Polistes wasps as a model system where we experimentally perturb the social structure by removing the queen and follow the re-establishment of the social steady state over time. We combine a unique experimental strategy correlating time-resolved measurements across vastly different scales with a theoretical approach. We show that Polistes integrates antagonistic processes on multiple scales to distinguish between extrinsic and intrinsic perturbations and thereby achieve both robust specialisation and rapid plasticity. The long-term stability of the social structure relies on dynamic DNA methylation which controls transcriptional noise. Such dynamics provide a general principle of how both specialization and plasticity can be achieved in biological systems. Overall design: All individuals from Polistes canadensis nests were collected from the area of Puta Galeta, Colon (9°21'30.877''N, 79°54'0.0053''W). Characterisation of individual brain transcriptome was performed using RNA-sequencing. Individuals were collected under three conditions: 1/ Control (unmanipulated nests), 2/Queen removal (early commitment) (queen was removed from the nest and individuals were collected 4-5 days after the emergence of a new queen) and 3/ Queen removal (late commitment) (queen was removed from the nest and individuals were collected 2 weeks after the emergence of a new queen).