Whole exome sequencing of mouse model of therapy-related myeloid neoplasms

The genetic profile of therapy-related myeloid neoplasms ( t-MN) is markedly skewed towards high-risk cytogenetic and molecular abnormalities, and complex karyotypes with a del(5q), and TP53 mutation/loss are profoundly over-represented in t-MN as compared with de novo counterparts. Concurrent haploinsufficient expression of the del(5q) tumor suppressor genes, early growth response 1 (EGR1) and adenomatous polyposis coli (APC), cooperate with TP53 (p53) loss to induce myeloid leukemia in mice. Whole exome sequencing was performed on bone marrow isolated from mice that developed MDS or AML.