Single cell RNA-seq of a laser treated alopecia model

Hair loss, including alopecia, is one of the most common dermatological issues worldwide. At present, the application of fractional CO2 laser in the treatment of alopecia has been few documented, however, the results are varied between the reports. These different results may come from limited knowledge of cellular action in the laser irradiated skin. The objective of this study was to investigate the molecular and cellular mechanism of laser treatment at the affective condition for hair cycle initiation. Overall design: Fractional CO2 laser was applied and optimized to initiate their hair cycle in a mouse model of alopecia. The irradiated skin was analyzed by multiple cellular markers with immunofluorescent staining and flow cytometry. The cellular populations and their comprehensive gene expression was analyzed by single cell RNA sequencing and bioinformatics.