AB* zebrafish embryos exposed to 1 ng/mL TCDD from 1d to 5d post-fertilization.

Transcriptomic profiling of the response to dioxin in developing zebrafish embryos, at 24, 48, 72, 96, and 120 h post-fertilization. The goal was to elucidate mechanisms by which dioxin causes toxicity in zebrafish; this dose was previously shown to induce teratogenesis. Two conditions (dioxin or DMSO exposure), 5 timepoints, 4 replicates per timepoint derived from 4 entirely separate experiments; multiple pooled embryos per replicate. Embryo RNA was compared on each chip to a universal reference RNA obtained from zebrafish Zf4 cells (embryonic fibroblast cells).