Using Illumina HiSeq 2500 platform with dual-indexed paired-end to sequence the hotspring sample. Metagenomic sequencing hotspring sample

A comprehensive investigation of the phylogenetic composition and metabolic potential of Sungai Klah, which is located in Perak, Malaysia. It is the second hottest geothermal springs in Malaysia. The hotspring is a streamer-like and several spring heads with boiling temperature (100-110 °C) are presence. Other parts of the streaming water exhibit the temperature ranging from 50−100 °C. Little knowledge on the types of hyper- and thermophilic within this hot spring is known. Furthermore, this high temperature environment condition serves as a source for understanding the genetic diversity, population structure, and ecological roles of these boiling point-tolerant microorganisms.