Additional file 3: of iGUIDE: an improved pipeline for analyzing CRISPR cleavage specificity

Table S3. Evidence for mispriming: sequence reads recovered from control reactions containing DNA from T cells that were not treated with the double-stranded oligonucleotide (ODN). We note that additional mispriming events in cells that were transfected with the ODN may have a different character. Inspection of data suggests formation of chimeric PCR products, probably involving DNA chains amplified by priming on the ODN initially, which go on to form complex molecules that under some circumstances map in a fashion paralleling simple mispriming. The iGUIDE method filters out many of these more complex artifacts as well. (XLS 21 kb)