Description of Changes in the Metabolic Profile of hCMEC/D3 cells (BBB Model) under treatment with vasoactive peptides

Metabolomic studies are one of the most informative tools in comparing different stimulation conditions of biological models. In this paper, a metabolomic analysis and comparison was performed in hCMEC/D3 cell groups cultured under treatment conditions with Angiotensin II (Ang-II) and Angiotensin 1-7 (Ang 1-7). The results are insightful for further exploration of the pathways associated with the metabolomic response observed. Our scope was to describe the metabolomic patterns present in the cells treated with the aforementioned vasoactive peptides to elucidate probable pathways associated with cellular responses to their activity. hCMEC/D3 cultured cells were stimulated with Ang-II, and Ang 1-7 in the log phase and the metabolomic analysis was performed with HPLC-MS and GC-MS. Interesting differences were found in the metabolomic profile of the treated cells. More than 80 substances were found to be expressed differentially between the groups. High differences were observed in the expression of metabolites associated with fatty acyls and glycerophospholipid pathways. The preliminary results in this study are insightful to recognize several metabolic pathways associated with stress responses in the treated cells. Further research is needed to explore the mechanisms of these pathways in the physiological response of the BBB in the presence of vasoactive peptides