Proteome of the cellulose degradation system of Cytophaga hutchinsonii and Sporocytophaga myxococcoides

Both C. hutchinsonii and S. myxococcoides were grown on filter paper. Time points were taken in both early and late stages of growth to assess the cellulolytic proteins utilized during growth. As a non-cellulose control, the organisms were grown on pectin as sole carbon source. Further, the cells were also fractionated to seperate the cellular fractions in order to assess the localization of the proteins within the growing cells.