Gene expression in human bone marrow Mesenchymal Stem cell line (hMSC-TERTs) during proliferation, quiescence (G0-arrest) and re-activation.

A microarray study using human Mesenchymal Stem cell line (hMSC-TERTs), was designed in order to detect the differences in global gene expression during proliferation, quiescence (G0) and reactivation post quiescence for 1 hr (R1), 3 hrs (R3) and 24 hrs (R24). Proliferating and re-activated cells were harvested at 70-75% confluency. To induce quiescence, MSCs were cultured in suspension in methyl cellulose in presence of full serum complement. The raw data from gene arrays were normalized and the five samples were compared to each other in order to detect differences in gene expression between the samples. Asynchronously proliferating hMSCs-TERTs was cultured in two different culture conditions, in adherent condition (proliferating) and in non-adherent suspension condition (quiescent). Gene expression profiling of proliferating, quiescent and re-activated hMSCs was performed using agilent arrays. All the samples were taken in triplicates. Thus the numeber of samples for microarray analysis was 15.