Characteristics of enzymes used in network construction.
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Substrate for each enzyme should fulfill all the required conditions. For the specific description of glycosidic linkages, refer to glycan structures in Figure 1. ManI: mannosyl-oligosaccharide 1,2-α-mannosidase; ManII: mannosyl-oligosaccharide 1,3-1,6-α-mannosidase; GnTI: α-1,3-mannosyl-glycoprotein 2-β-N-acetylglucosaminyltransferase; GnTII: α-1,6-mannosyl-glycoprotein 2-β-N-acetylglucosaminyltransferase; GnTIII: β-1,4-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase; GnTIV: α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase; GnTV: α-1,6-mannosyl-glycoprotein 6-β-N-acetylglucosaminyltransferase; FucT: glycoprotein 6-α-L fucosyltransferase; GalT: β-N-acetylglucosaminylglycopeptide β-1,4-galactosyltransferase; SiaT: β-galactoside α-2,3/6-sialyltransferase.
