Genome wide CRISPR screen for Venetoclax resisatnce in MOLM13 cells using Brunello library. Genome wide CRISPR screen for Venetoclax resisatnce in MOLM13 cells using Brunello library

MOLM13 cells were infected with CRISPR/Cas9 library, selected for puromycn resistance for integration events and exposed to Venetoclax or vehicle, DMSO, at 1microMolar concentrations. Cells were collected at time 0 (post puromycin selection), day 7 and 14 , DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Hiseq using 6 samples per lane. Overall design: perform pairwise comparisons between sgRNA distributions at initial time point (DMSO day 0) and plasmid, between DMSO and Venetoclax treated cells at corresponding time points.