Genome sequencing with short reads

To test methods for assembly of very short reads, five microbial genomes were selected which have a finished reference sequence available. Escherichia coli MG1655 was one of these five microbial genomes. At least two libraries (short fragment and jumping) were generated and sequenced using Illumina. Likewise in particular for E. coli, libraries were also generated and run for 454 sequencing. Genomic DNA from Erica Sodergren. Two different genome assemblies are part of this project. The assembly in AKBV00000000 is the complete chromosome assembled from Illumina reads. The assembly in AKVX00000000 is the complete chromosome assembled from reads used to assemble U000096 plus reads from a different isolate of the strain.