Transcriptional variability analysis, GSEA, and TF motif enrichment analysis of a T21 dataset.

Sheet 1: Change in VMR for each gene between control and T21 mouse model (Dp1Tyb) conditions. Sheet 2: DEGs between control and T21 conditions. Sheet 3: Top 100 DEGs by absolute fold change and top 100 genes with largest absolute change in VMR, as well as the genes that are contained in both lists. All comparisons are between the control and T21 mouse model (Dp1Tyb) conditions. Sheet 4: KEGG pathways resulting from GSEA using the 1000 most significant DEGs, ranked by log-fold change versus from GSEA using the top 1000 genes with largest absolute change in VMR, ranked by change in VMR. Sheet 5: Enriched motifs among the 100 most significant DEGs, and among the top 100 genes with largest absolute change in VMR. Each motif has a normalized enrichment score (NES), AUC score, predicted TFs, number of enriched genes, and enriched genes. Sheet 6: Predicted enriched TFs from the motifs found in Sheet 5, after filtering out TFs that are not expressed in neither the control nor T21 conditions. Enriched TFs that are only enriched among the top 100 genes with largest absolute change in VMR, and not among the 100 most significant DEGs, are also listed. (XLSX)