The Mre11 nuclease activity is required for TLS.
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Single and double mutants were transformed with plasmids carrying wild type, the nuclease/helicase-dead mutations or the vector alone. Overnight cell cultures were imprinted on YPD or YPD+MMS gradient plates at desired concentrations and incubated at 30°C for 2 days before being photographed. Yeast strains used: DBY747 (wild type), WXY2379 (mre11Δ) and WXY2390 (mre11Δ rev3Δ). All strains are isogenic to DBY747.
创建时间:
2016-02-23



