Gene expression profiling of human-derived primary cardiomyocytes with lncRNA ZNF593-AS 5' end overexpression under phenylephrine stimulation

The aim of this study is to investigate molecular mechanisms underlying the function of lncRNA ZNF593-AS in cardiomyocyte under phenylephrine stimulation in vitro. Overall design: Human-derived primary cardiomyocytes were transfected with pcDNA3.1 or ZNF593-AS 5' end, the functional fragment of ZNF593-AS under the stimulation of phenylephrine. We extracted the total RNA 48h after the transfection and perform RNA-seq using Illimina platform.