Label free mass spectrometry quantification of Akt interacting proteins in insulin stimulated cardiomyocytes from Metabolic Syndrome Rats.

In the present work, we used a label-free mass spectrometry approach to identify changes in the abundance of Akt-interacting proteins in cardiomyocytes of MetS rats. Using this strategy, we were able to identify specific proteins with evident dissimilarities in their abundance within the Akt complex. Our data provide for the first time proteomic support to the documented impairment in energy metabolism and insulin signaling in the heart as a consequence of the MetS condition.