The role of NLRP3 in intra-amniotic inflammation-induced preterm birth

Macrophages and neutrophils were sorted from the decidua and uterus of Nlrp3+/+ and Nlrp3-/- mice injected with lipopolysaccharide (LPS) or PBS and analyzed using pair-end RNA-sequencing (RNA-seq) to evaluate the effect of Nlrp3 deficiency on the transcriptomes of these innate immune cells in preterm birth. The study included Nlrp3+/+ and Nlrp3-/- dams intra-amniotically injected with lipopolysaccharide (LPS) or PBS. Decidual and uterine macrophages and neutrophils were sorted by fluorescence-activated cell sorting. The number of samples analyzed were as follows: Macrophages, n = 3 per group; Neutrophils, n = 2 per group. Please note that C57BL/6 and B6.129S6-Nlrp3tm1Bhk/J strains were used for the study