Supporting data for “Salt-inducible Kinase 2 : A Potential Metastasis Suppressor in Hepatocellular Carcinoma”

Supporting data for the MPhil thesis titled "Salt-inducible Kinase 2 : A Potential Metastasis Suppressor in Hepatocellular Carcinoma". SIK2, a serine/threonine kinase under the AMP-activated protein kinase (AMPK) family, was found to play an important role in the pathogenesis of various cancers affecting the growth, mobility, invasion, and chemosensitivity of cancer cells. However, the function of SIK2 in hepatocellular carcinoma (HCC), one of the leading causes of cancer mortality around the world, has not yet been identified. Therefore, by establishing SIK2 knockout cell lines through the CRISPR/Cas 9 tools, this study explored the role of SIK2 in HCC. Depletion of SIK2 affected proliferation, metastatic properties, and chemoresistance of HCC cells. This dataset includes images taken from western blot for knockout and transfection confirmation, immunohistochemistry on clinical HCC samples, in vitro cell assays and in vivo animal experiment assessing the cell functions of SIK2 knockout cells, with corresponding quantification data.

本数据集支持名为《盐诱导激酶2：肝细胞癌中潜在的转移抑制因子》的哲学硕士论文。SIK2，作为一种丝氨酸/苏氨酸激酶，属于AMP激活蛋白激酶（AMPK）家族，研究发现其在多种癌症的发生发展中起着至关重要的作用，影响癌细胞的生长、迁移、侵袭和化疗敏感性。然而，SIK2在肝细胞癌（HCC）这一全球癌症死亡率的主要原因中的功能尚未明确。因此，本研究通过CRISPR/Cas 9技术构建SIK2敲除细胞系，探讨了SIK2在HCC中的作用。SIK2的缺失影响了HCC细胞的增殖、转移特性和化疗耐药性。本数据集包括来自敲除和转染验证的western blot图像、临床HCC样本的免疫组化分析、体外细胞实验以及评估SIK2敲除细胞功能的体内动物实验的图像，并附有相应的量化数据。