Sema3d restrained Hepatocellular carcinoma progression through inactivating Pi3k/Akt signaling via interact with FLNA. Sema3d restrained Hepatocellular carcinoma progression through inactivating Pi3k/Akt signaling via interact with FLNA

Purpose: High throughput RNA-seq has provided an efficient and convenient access to screening substantial differentially expressed genes (DEGs). The goals of this study are to screen out Sema3d-regulated DEGs of HCC cells by utilizing RNA-sequencing and to furtherly identify specific biological function-related gene subset from Sema3d-regulated DEGs. Method: Firstly, HCCLM3 cells were seperated into two groups and respectively transfected with lentivirus-delivered Sema3d and control. We cultured these cells with DMEM containing 10% FBS serum. 48 hours later, cells of two groups were harvested by Trizol RNA isolater and examined expression difference of Sema3d, and then analyzed by High througput RNA-sequencing. Conclusion: Using next generation high throughput RNA-sequencing technology and using |log2(FoldChange)|>1 and Qvalue1 and Qvalue<0.05 as a cutoff for statistic analysis.