Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex

This dataset contains the structural coordinates and corresponding cryo-EM density maps associated with the manuscript “Recognition and Remodelling of Nucleosomes and Hexasomes by the Human INO80 Complex”. Specifically, it includes: · INO80–Hexasome Complexes: Structures and maps of the human INO80 complex (core module) bound to 0H80 hexasomes (PDB: 9GE5, EMDB: 51290) and the Hexasome structure (PDB: 9GEL, EMDB: 51294). Both are in the presence of ADP–BeF₃. · INO80–Nucleosome Complexes: Structures and maps of the complex (core module) bound to 50N50 H2A.Z-containing nucleosomes (PDB: 9GCG, EMDB: 51229) and the H2A.Z containing nucleosome (PDB: 9GE4, EMDB: 51289).and 0N40 nucleosomes in two distinct binding states (N6 and N7). The INO80–nucleosome complex in state N-6 includes INO80-Nucleosome full structure state N-6 (PDB: 9GEV, EMDB: 51307) and Nucleosome state N-6 (PDB: 9GF6, EMDB: 51310). The INO80–nucleosome complex in state N-7 includes INO80-Nucleosome full structure state N-7 (PDB: 9GFB, EMDB: 51312) and Nucleosome state N-7 (PDB: 9GFM, EMDB: 51313). All are in the presence of ADP–BeF₃. In addition, three more cryo-EM maps of the human INO80 (core and arp module) complex bound to nucleosomes or hexasomes are included: · EMDB: 53574 – Cryo-EM map of the human INO80 complex bound to a nucleosome (0N40) in the absence of nucleotide. · EMDB: 53579 – Cryo-EM map of the human INO80 complex bound to a hexasome (0H80) in the presence of ADP–BeF₃. · EMDB: 53580 – Cryo-EM map of the human INO80 complex bound to an H2A.Z nucleosome (50N50) with the A-module contacting both sides of extranucleosomal DNA, in the presence of ADP–BeF₃. Together, these datasets provide a comprehensive structural resource for understanding how the human INO80 complex recognizes and remodels nucleosomal substrates in different configurations.