RNA sequencing of DU145 (a human prostate cancer cell line) NEK9 genetically Knocked out cell line compared to its wildtype parental counterpart

In this study, we performed a CRISPR-based kinome screening in multiple cancer cell lines and identified Never In Mitosis Gene-A Related Kinase-9 (NEK9) as a major player of Docetaxel resistance in OSCC, prostate, and pancreatic cancer lines. We wanted to perform RNA sequencing to understand the possible mechanistic reason for drug sensitization.