How a paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody

Paramyxoviruses including measles, Nipah, and parainfluenza viruses are public health threats with pandemic potential. Human parainfluenza virus type 3 (HPIV3) is a leading cause of illness in pediatric, elderly, and immunocompromised populations. There are no approved vaccines or therapeutics for HPIV3. Neutralizing antibodies that target viral fusion are a potential strategy for mitigating paramyxovirus infection, however their utility may be curtailed by viral evolution that leads to resistance. Paramyxoviruses enter cells by fusing with the cell membrane in a process mediated by a complex consisting of a receptor binding protein (HN) and a fusion protein (F). Existing atomic resolution structures fail to reveal physiologically relevant interactions during viral entry. We present cryo-ET structures of pre-fusion HN-F complexes in situ on the surfaces of authentic virions that evolved resistance to an anti-HPIV3 F neutralizing mAb. Single mutations in F abolish mAb binding and neutral..., , , # Data from: How a paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody

[https://doi.org/10.5061/dryad.g1jwstqz6](https://doi.org/10.5061/dryad.g1jwstqz6)

Excel file including the raw data for the article \"How a Paramyxovirus Fusion/Entry Complex Adapts to Escape a Neutralizing Antibody.\"

## Description of the data and file structure

We have submitted our raw data for figures 1,2,3, and 5 (Hats_Off_Paper_Raw_Data_Final.xlsx), plasmid sequences encoding HN, HA, and F (Sequences.xlsx), and uncropped western blot surface expressions of F variants using anti-HPIV3 F HRC

### Descriptions

**Hats_Off_Paper_Raw_Data_Final.xlsx**

In Hats_Off_Paper_Raw_Data_Final.xlsx each tab contains the raw data and is labelled with the figure panels used in the manuscript: How a Paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody.

·      HN: hemagglutinin-neuraminidase protein

·      F: Fusion protein

·      HA: Uncleaved influenza hemagglutinin

·     ...