CIL:37052, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

This is IMAGE #3 (4 hours) of a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. Complete z-stacks were acquired of this neuron at a 2 hour interval over 8 hours. These images are part of this image group. This time lapse series shows the development of the neuron's dendritic arbor. The dendritic arbor exhibits morphological plasticity with branch additions, retractions and extensions. The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.