Large scale analysis of fruit evolution during cold storage within different apple varieties and HIVW progeny. Malus domestica

Based on sensorial analysis over 2 years, 12 apple varieties and 4 hybrides with contrasted fruit texture were selected for transcriptionnal analyses over 4 months of cold storage (harvest, 1 month, 2 and 4 months). Overall design: The 16 genotypes were associated in 8 pairs to perform competitive hybridisations of fruit samples at each time point (harvest, 1, 2 and 4 months cold storage) from the same harvest year. A total of 44 comparisons including dye-swap repetitions were performed. Biological replicates are fruits from 2 to different harvest year. 4 additionnal comparisons were performed between 'harvest' and '2 months cold storage' for 2 genotypes from 2 different harvest year (biological replicates), including dye-swap (technical replicates).