Gene expression profiling of LNCaP, LNCap-abl and 22Rv1 cells following transfection with toxic siRNAs (siGUGUGUA, siUGUUUGC), siAR or non-target control as indicated

Analysis of RNA expression in LNCaP and 22Rv1 cells following transfection with siUGUUUGC or siGUGUGUA siRNAs. 22Rv1 cells were also transfected with siRNA targeting AR as a positive control for reduced AR transcriptional activity. We report global changes in AR regulated gene expression expression. Overall design: 22Rv1 cells were grown in RPMI supplemented with 10% charcoal-stripped FBS (CSS) in the presence (+ ) or absence of dyhidrotestosterone (DHT) for six days prior to siRNA transfectios. LNCaP cells were maintained in RPMI supplemented with 10% FBS. LNCaP-abl were grown in RPMI suplemented with 10% CSS. RNA was extracted 40 hrs, 48 hrs and 64 hrs after siRNA transfections in LNCaP, LNCap-abl and 22Rv1 cells, respectively . Each sample was run in tiplicate.