Transcription profiling of mouse C57/B6 multistage liver development

The liver performs a number of essential functions for life. The development of such a complex organ relies on finely regulated gene expression profiles which change over time in the development and determine the phenotype and function of the liver. We used high-density oligonucleotide microarrays to study the gene expression and transcription regulation at 14 time points across the C57/B6 mouse liver development, which include E11.5 (embryonic day 11.5), E12.5, E13.5, E14.5, E15.5, E16.5, E17.5, E18.5, Day0 (the day of birth), Day3, Day7, Day14, Day21, and normal adult liver. With these data, we made a comprehensive analysis on gene expression patterns, functional preferences and transcriptional regulations during the liver development. Experiment Overall Design: Totally 25 microarrays have been used in this experiment, and 11 of the 14 time points have two technique repeat experiments.肝脏是维系生命运转的核心器官之一。这一高度复杂器官的发育依赖于精密调控的基因表达谱，该表达谱随发育进程动态改变，并决定肝脏的表型与功能。本研究采用高密度寡核苷酸微阵列（high-density oligonucleotide microarrays），对C57/B6小鼠肝脏发育过程中的14个时间节点开展基因表达与转录调控分析，涵盖胚胎第11.5天（E11.5）、E12.5、E13.5、E14.5、E15.5、E16.5、E17.5、E18.5、出生当日（Day0）、出生后第3天（Day3）、第7天（Day7）、第14天（Day14）、第21天（Day21）以及正常成年肝脏。基于上述数据集，我们对肝脏发育过程中的基因表达模式、功能偏好性及转录调控机制进行了全面解析。实验整体设计：本实验共使用25张微阵列芯片，14个时间节点中有11个节点设置了2次技术重复实验。