Comparison the transcriptional changes in the mutation of VZV circVLTs

Sequence analyses shown that most exons of VZV circVLTs were overlapped with ORFs, except for exon5, which was an ideal mutation position. Using a galK positive/counter selection VZV bacteria artificial chromosome (BAC) system, we replaced upstream (104,765-104,785 Mutation2, pOKA-M2) or downstream (104,785-104,805, Mutation1, pOKA-M1) of circVLTs BSJ exon5 with 20 bp random sequences in VZV genomic DNA level. The code used in this study and extended data are available from the GitHub repository (https://github.com/ShaominYang/VZV_circRNA) ARPE-19 cells were infected with VZV pOKA-WT, pOKA-M1 and pOKA-M2. RNA-seq was used to quantify the expression of cellular and viral genes