TPM-with-cluster_updated 5825.csv

The carotid body (CB) chemoreceptors mediate rapid cardiorespiratory responses to hypoxia, which maintain systemic oxygen homeostasis, but CB dysfunction is also implicated in pathologies including hypertension, heart failure and sudden infant death.CB-mediated chemoreflexes mature during the perinatal period, with increasing sensitivity of the oxygen chemosensory response.We performed RNA-seq of CBs from sheep across 3 perinatal stages and adults, enabling us to identify gene expression changes that correlate with functional state. In parallel, we also analysed superior cervical ganglion (SCG) tissue at the same stages as an oxygen-insensitive control. n=3 biological replicates per stage, except for one d145 SCG that was omitted due to RNA quality.We then performed hierarchical clustering analysis on all genes showing significant differential expression between CB and SCG at any time point. This yielded 6 clusters of genes with different tissue-specific, temporal variation in expression across developmental time: three comprising genes that are CB-enriched (C1, C2, C3) and three with SCG-enriched genes (S1, S2, S3). Cluster C1 and S1 genes are predominantly enriched at early/fetal time points, while C3 and S3 genes increase with development and maturation. Genes in clusters C2 and S2 show more complex, intermediate temporal patterns of expression.The attached file shows expression levels (tpm) for a given gene in each sample and the overall cluster of that gene (if applicable).