RNA-seq based transcriptomic profiling of GBM44 glioma cell line with BAT1 shRNA knockdown

Purpose: We found that BAT1 plays key role in the regulation of immune related factors, we aim to characterize the genes changed after BAT1 knockdown Methods: Gene expression profile of GBM44 control and BAT1 knockdown were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000 Results: Using an optimized data analysis pipeline(HISAT2/HT-Seq/DESeq2), 5209 differentially expression protein-coding and noncoding genes (cut-off adjusted P <0.01) were identified in response to BAT1 knockdown. Conclusions: After BAT1 knockdown, thousands of genes are differentially regulated, and these genes participate in immuse defense, Inflammatory response as well as cell migration and proliferation (GO pathway analysis). Gene expression profile of GBM44 control and BAT1 knockdown were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000