Transcriptome comparisons of "expandable hemangioblasts" (eHBs) and their progeny to control cells

We examined the transcriptomes of murine "expandable hemangioblasts" (eHBs) and their blood and endothelial progeny, comparing them to the transcriptomes of murine embryonic stem (ES) cells, primary murine endothelial cells isolated from E11.5 yolk sacs or embryos, and E14.5 fetal liver hematopoietic stem cells. Total RNAs were purified from lysates of cultured or primary cells, reverse transcribed, and sequenced on an Illumina HiSeq 2500.我们对小鼠可扩增成血管母细胞（expandable hemangioblasts, eHBs）及其血液与内皮子代细胞的转录组进行了分析，并将其与小鼠胚胎干细胞（embryonic stem, ES cells）、从胚胎发育第11.5天（E11.5）卵黄囊或胚胎中分离的原代小鼠内皮细胞，以及胚胎发育第14.5天（E14.5）胎肝造血干细胞的转录组进行了对照比较。我们从培养细胞或原代细胞的裂解液中纯化总RNA，经反转录后在Illumina HiSeq 2500测序平台上完成了测序。