Figure 7B

<b>A.</b> MRC5 cells were plated on coverslips and infected with TB40/E-mCh), UL88-STOP-mCh, or UL88-Rev-mCh at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue) and NF-KB (green), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.<b> B. </b>MRC5 cells expressing either GFP-vector or GFP-UL88 were plated on coverslips and infected with mCherry tagged WT or UL88-STOP TB40/E virus at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue), GFP (green) and NF-KB (grey), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.