Relative expression of sulfate assimilation genes in fou8.

Col-0 and fou8 plants were grown for 5 weeks in controlled environment room. Total RNA was isolated from leaves and the transcript levels of genes involved in sulfur metabolism was determined by quantitative RT-PCR. In two independent experiments qRT-PCR reactions were performed in triplicate for each of the three independent biological samples. The values in Col-0 were set to 1 for all genes. Results of one experiment are presented as means ± SD from three pools of three individual plants. P-values obtained by Student's T-test are also given.