An in vitro human liver model by iPSC-derived parenchymal and non-parenchymal cells

We have established culture systems to generate liver progenitor cells (LPCs), liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs) from hiPSCs. Then, we established co-culture system of hiPSC-derived liver cells and performed RNA-seq of hiPSC-derived liver model. Overall design: Liver progenitor cells (LPCs), Liver sinusoidal endothelial cells (LSECs, and hepatic stellate cells (HSCs) were generated from human iPS cells. Expression profile of hiPSC-derived liver model (co-culture system of LPCs and NPCs, N=2) was compared with hiPSC-derived LPCs (N=2) and primary human hepatocytes (N=2) by RNA-seq.