Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

Raw multichannel and/or Z-stack source data from time series images in TIF format to accompany publication of:

Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

Jina Ko1, Martin Wilkovitsch2, Juhyun Oh1, Rainer Kohler1, Evangelia Bolli1,3, Mikael J. Pittet1,3,4,5, Claudio Vinegoni1, David B. Sykes6,7, Hannes Mikula2, Ralph Weissleder1,8*, Jonathan C. T. Carlson1,7*

1 Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114 

2 Institute of Applied Synthetic Chemistry, TU Wien, 1060 Vienna, Austria 

3 Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland

4 Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland

5 AGORA Cancer Center, Lausanne, Switzerland

6 Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA

7 Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA

8 Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115