RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in the abscence of lsy-2 activity

lsy-2 encodes in C. elegans a C2H2 zinc-finger transcription factor involved in the specification of the left-right asymmetry of the ASE neurons (Johnston and Hobert, 2005) and in the maintenance of germ-soma distinction (Lin et al., 2015). RNA for three different biological replicates was isolated from at least 1000 animals of each genotype: wild type and lsy-2(ot64) and then sequence to detect differentially expressed genes in lsy-2 mutant genotype compared to wild type animals.